Mouse Keratinocyte Growth Factor(KGF/FGF-7) ELISA Kit

Mfr.No.

CSB-E13046m

Sensitivity

0.39 pg/mL

Detection Range

1.56 pg/mL-100 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

fibroblast growth factor 7 (keratinocyte growth factor), HBGF-7, KGF, fibroblast growth factor 7, heparin-binding growth factor 7, keratinocyte growth factor

Recovery

The recovery of human ELTD1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	101	98-104
EDTA plasma (n=4)	87	82-91

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ELTD1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	94
	Range %	89-98
1:2	Average %	97
	Range %	91-103
1:4	Average %	92
	Range %	87-98
1:8	Average %	88
	Range %	85-92

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1600	2.116	2.231	2.174	2.012
800	1.597	1.643	1.620	1.458
400	1.166	1.187	1.177	1.015
200	0.833	0.826	0.830	0.668
100	0.551	0.563	0.557	0.395
50	0.362	0.372	0.367	0.205
25	0.281	0.287	0.284	0.122
0	0.161	0.162	0.162	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P36363

Applications	ELISA
Research Area	FGF7

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10506099	CSB-E13046m	96T	$790	Add to Cart
BHE10506099	CSB-E13046m	5x96T	$3792	Add to Cart
BHE10506099	CSB-E13046m	10x96T	$7505	Add to Cart

Mouse Keratinocyte Growth Factor(KGF/FGF-7) ELISA Kit

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