Mouse Keratin, type I cytoskeletal 18 fragment (K18-Asp396)ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E14265m

Sensitivity

3.9 uIU/mL

Detection Range

15.6 uIU/mL-1000 uIU/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Cytokeratin 18-M30(CK 18-M30)

Recovery

The recovery of human OT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-96
EDTA plasma (n=4)	96	90-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human OT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	89
	Range %	85-96
1:2	Average %	95
	Range %	91-99
1:4	Average %	98
	Range %	92-103
1:8	Average %	93
	Range %	87-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	CK 18-M30

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Pilot study of the antifibrotic effects of the multikinase inhibitor pacritinib in a mouse model of liver fibrosis Suliman Al-Fayoumi.et al,J Exp Pharmacol.,May 9

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