Matrices listed below were spiked with certain level of IRF7 and the recovery rates were calculated by comparing the measured value to the expected amount of IRF7 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-103
92
EDTA plasma(n=5)
86-102
95
heparin plasma(n=5)
85-105
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IRF7 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-102%
85-104%
86-98%
EDTA plasma(n=5)
83-91%
84-99%
82-100%
heparin plasma(n=5)
85-90%
80-100%
80-92%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
P70434
Applications
ELISA
Applications Notes
IRF7 ELISA Kit allows for the in vitro quantitative determination of IRF7 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse IRF7(Interferon regulatory factor 7) ELISA Kit