Mouse Interleukin 13 - IL-13 ELISA Kit

Mfr.No.

E0019Mo

Description

This sandwich kit is for the accurate quantitative detection of Interleukin 13 (also known as IL-13) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

IL 13;IL13;IL-13;Interleukin-13;T-cell activation protein P600

Reactivity

Mouse

Detection Range

1-400ng/L

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

14108

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.52ng/L

Background

Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses (By similarity). Positively regulates IL31RA expression in macrophages (PubMed: 25847241).

Gene ID	16163
UniProt ID	P20109
Antigen	IL13

Research Area

Immunology

Protocol

These standard curves of E0019Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
200ng/L	2.253	2.261	2.223
	2.268
100ng/L	1.395	1.347	1.309
	1.299
50ng/L	0.739	0.675	0.637
	0.611
25ng/L	0.363	0.359	0.321
	0.354
12.5ng/L	0.148	0.177	0.139
	0.206
0ng/L	0.027	0.038	0
	0.048

Precision

We measured random samples of E0019Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	104.8	3.48	3.3
Intra-Assay	2	18	68.6	3.84	5.6
Intra-Assay	3	18	33.1	1.58	4.8

Storage	2-8°C

Reference	Effect of Coal Dust Exposure on the SOD Activity and the Oxidative DNA Damage in Asthmatic Mice Journal: Indones Biomed J. 2019; 11(2): 159-66

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107947	E0019Mo	48T	$320		Add to Cart
BHE12107947	E0019Mo	96T	$458		Add to Cart

Mouse Interleukin 13 - IL-13 ELISA Kit

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