Mouse Interleukin 1 receptor antagonist, IL-1ra ELISA kit

Mouse Interleukin 1 receptor antagonist, IL-1ra ELISA kit

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Mfr.No.

CSB-E10395m

Sensitivity

15.6 pg/mL

Detection Range

62.5 pg/mL-4000 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

interleukin 1 receptor antagonist, DIRA, ICIL-1RA, IL-1ra3, IL1F3, IL1RA, IRAP, MGC10430, MVCD4, IL1RN (IL1F3), OTTHUMP00000203730, intracellular IL-1 receptor antagonist type II, intracellular interleukin-1 receptor antagonist (icIL-1r

Recovery

The recovery of grouper VTG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-100
EDTA plasma (n=4)	95	91-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of grouper VTG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	84-97
1:2	Average %	97
	Range %	91-102
1:4	Average %	95
	Range %	88-103
1:8	Average %	96
	Range %	91-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
2000	0.174	0.175	0.175
1000	0.359	0.378	0.369
460	0.567	0.552	0.560
150	0.872	0.891	0.882
50	1.513	1.476	1.495
0	2.389	2.411	2.400

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P25085

Applications	ELISA
Research Area	IL1RN

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10506036	CSB-E10395m	96T	$595	Add to Cart
BHE10506036	CSB-E10395m	5x96T	$2856	Add to Cart
BHE10506036	CSB-E10395m	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.