Mouse interferon-inducible protein 10,IP-10 ELISA Kit

Mouse interferon-inducible protein 10,IP-10 ELISA Kit

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Mfr.No.

CSB-E08183m

Sensitivity

0.78 ng/mL

Detection Range

0.312 ng/ml - 20 ng/ml

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

chemokine (C-X-C motif) ligand 10, C7, IFI10, INP10, IP-10, SCYB10, crg-2, gIP-10, mob-1, gamma IP10, interferon-inducible cytokine IP-10, protein 10 from interferon (gamma)-induced cell line, small inducible cytokine B10, small inducibl

Recovery

The recovery of pig eNOS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-92
EDTA plasma (n=4)	100	97-103

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig eNOS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	83
	Range %	80-86
1:2	Average %	97
	Range %	93-100
1:4	Average %	84
	Range %	80-88
1:8	Average %	104
	Range %	100-108

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

IU/ml	OD1	OD2	Average	Corrected
200	2.589	2.547	2.568	2.386
100	1.829	1.809	1.819	1.637
50	1.064	1.087	1.076	0.894
25	0.643	0.657	0.650	0.468
12.5	0.450	0.468	0.459	0.277
6.25	0.358	0.369	0.364	0.182
3.12	0.274	0.298	0.286	0.104
0	0.181	0.183	0.182

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P17515

Applications	ELISA
Research Area	CXCL10

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10505996	CSB-E08183m	96T	$695	Add to Cart
BHE10505996	CSB-E08183m	5x96T	$3336	Add to Cart
BHE10505996	CSB-E08183m	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.