Mouse Interferon Gamma - IFN-G ELISA Kit

Mfr.No.

E0056Mo

Sensitivity

2.43ng/L

Detection Range

5-1000ng/L

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Interferon γ (also known as IFN-γ) in serum, plasma, cell culture supeates, cell lysates, tissue homogenates.

Alternative Names

IFN G;IFN gamma;IFNG;IFN-gamma;Interferon gamma

Molecular Weight

17907

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E0056Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	321.1	8.9	2.8
Intra-Assay	2	18	174.6	8.83	5.1
Intra-Assay	3	18	131.4	6.01	4.6

Background

Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

Protocol

These standard curves of E0056Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
640ng/L	2.269	2.213	2.16
	2.158
320ng/L	1.472	1.45	1.397
	1.429
160ng/L	0.772	0.784	0.73
	0.796
80ng/L	0.471	0.459	0.405
	0.446
40ng/L	0.306	0.287	0.234
	0.269
0ng/L	0.052	0.054	0
	0.055

Gene ID	15978
UniProt ID	P01580

Research Area	Immunology; Stem Cells; Cancer

Storage	2-8°C

Reference	Activation of the cannabinoid receptor 2 increases renal perfusion Joual: Physiol Genomics. 2019 Mar 1;51(3):90-96.

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107984	E0056Mo	48T	$320		Add to Cart
BHE12107984	E0056Mo	96T	$458		Add to Cart

Mouse Interferon Gamma - IFN-G ELISA Kit

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