Mouse Interferon beta,IFN-beta/IFNB ELISA Kit

Mfr.No.

CSB-E04945m

Alternative Names

interferon, beta 1, fibroblast, IFB, IFF, IFNB, MGC96956, interferon beta

Reactivity

Mouse

Detection Range

15.6 pg/mL-1000 pg/mL

Sample Type

Tissue homogenates, Cell lysates, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rat TN-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	88	84-92

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat TN-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	84
	Range %	80-88
1:4	Average %	106
	Range %	101-113
1:8	Average %	93
	Range %	89-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Sensitivity

3.9 pg/mL

Limit Of Detection

450 nm

UniProt ID	P01575
Antigen	interferon, beta 1, fibroblast

Applications

ELISA

Research Area

IFNB1

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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