Mouse Intercellular Adhesion Molecule 1

Mfr.No.

E0039Mo

Sensitivity

9.83ng/L

Detection Range

20-6000ng/L

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of intercellular adhesion molecule 1 (also known as ICAM-1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

CD antigen CD54;ICAM 1;ICAM1;ICAM-1;Intercellular adhesion molecule 1;MALA-2;MyD10

Molecular Weight

58844

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0039Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	663.1	43.67	6.6
Intra-Assay	2	18	1408	77.72	5.5
Intra-Assay	3	18	286.4	17.51	6.1

Background

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation (By similarity).

Protocol

These standard curves of E0039Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
3200ng/L	2.116	2.21	2.16
	2.303
1600ng/L	1.732	1.814	1.765
	1.897
800ng/L	1.203	1.221	1.171
	1.238
400ng/L	0.631	0.644	0.595
	0.658
200ng/L	0.439	0.431	0.382
	0.422
0ng/L	0.036	0.049	0
	0.063

Gene ID	15894
UniProt ID	P13597

Research Area	Immunology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107967	E0039Mo	48T	$320		Add to Cart
BHE12107967	E0039Mo	96T	$458		Add to Cart

Mouse Intercellular Adhesion Molecule 1 - ICAM-1 ELISA Kit

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