Mouse intercellular adhesion molecule 1,ICAM-1 ELISA KIT

Mouse intercellular adhesion molecule 1,ICAM-1 ELISA KIT

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Mfr.No.

CSB-E04575m

Alternative Names

intercellular adhesion molecule 1, BB2, CD54, P3.58, cell surface glycoprotein P3.58, human rhinovirus receptor, intercellular adhesion molecule 1 (CD54), human rhinovirus receptor

Reactivity

Mouse

Detection Range

78 ng/mL-5000 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rat CD73 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-101
EDTA plasma (n=4)	91	86-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat CD73 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	90
	Range %	87-96
1:2	Average %	87
	Range %	82-95
1:4	Average %	90
	Range %	85-97
1:8	Average %	93
	Range %	86-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mIU/ml	OD1	OD2	Average	Corrected
40	2.504	2.416	2.460	2.358
20	1.933	1.824	1.879	1.777
10	1.386	1.283	1.335	1.233
5	0.814	0.749	0.782	0.680
2.5	0.424	0.397	0.411	0.309
1.25	0.228	0.213	0.221	0.119
0.625	0.154	0.145	0.150	0.048
0	0.103	0.101	0.102	?

Species

Mus musculus

Sensitivity

19.5 ng/mL

Limit Of Detection

450 nm

UniProt ID	P13597
Antigen	Intercellular Adhesion Molecule 1

Applications

ELISA

Research Area

ICAM1

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10505989	CSB-E04575m	10x96T	$4446	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.