Mouse Insulin-like Growth Factor - Igf Binding Protein 5

Mfr.No.

E1500Mo

Sensitivity

0.22ng/ml

Detection Range

0.5-100ng/ml

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Insulin-like growth factor binding protein 5 (also known as IGFBP5) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

IBP-5;IGF-binding protein 5;IGFBP 5;IGFBP5;IGFBP-5;Insulin-like growth factor-binding protein 5

Molecular Weight

30372

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1500Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	23.7	1.06	4.5
Intra-Assay	2	18	46.9	1.93	4.1
Intra-Assay	3	18	11.4	0.56	4.9

Background

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Protocol

These standard curves of E1500Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
64ng/ml	2.242	2.129	2.071
	2.015
32ng/ml	1.361	1.389	1.331
	1.416
16ng/ml	0.764	0.763	0.705
	0.762
8ng/ml	0.408	0.41	0.352
	0.412
4ng/ml	0.296	0.269	0.211
	0.243
0ng/ml	0.059	0.058	0
	0.057

Gene ID	16011
UniProt ID	Q07079

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12109353	E1500Mo	48T	$320		Add to Cart
BHE12109353	E1500Mo	96T	$458		Add to Cart

Mouse Insulin-like Growth Factor - Igf Binding Protein 5 - IGFBP5 ELISA Kit

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