Mouse Insulin-like growth factor binding protein 4,IGFBP-4 ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E07355m

Sensitivity

0.195 ng/mL

Detection Range

0.78 ng/mL-50 ng/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

insulin-like growth factor binding protein 4, BP-4, HT29-IGFBP, IBP4, IGFBP-4, IGF-binding protein 4, insulin-like growth factor-binding protein 4

Recovery

The recovery of human TNFAIP3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	84-94
EDTA plasma (n=4)	99	95-103

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human TNFAIP3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-98
1:2	Average %	86
	Range %	82-92
1:4	Average %	95
	Range %	89-100
1:8	Average %	97
	Range %	94-106

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P47879

Applications	ELISA
Research Area	IGFBP4

Storage	Store at 2-8°C. Please refer to protocol.

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