Mouse Insulin-like 3(INSL3) ELISA kit

Mouse Insulin-like 3(INSL3) ELISA kit

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Mfr.No.

CSB-EL011747MO

Sensitivity

3.9 pg/mL

Detection Range

15.6 pg/mL-1000 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Alternative Names

insulin-like 3 (Leydig cell), MGC119818, MGC119819, RLF, RLNL, insulin-like 3, leydig insulin -like hormone, leydig insulin -like peptide, relaxin-like factor b

Recovery

The recovery of dog TM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	83-94
EDTA plasma (n=4)	93	87-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of dog TM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:10	Average %	97
	Range %	91-103
1:20	Average %	94
	Range %	91-98
1:40	Average %	90
	Range %	88-94
1:80	Average %	93
	Range %	89-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
1500	0.087	0.086	0.087
750	0.148	0.142	0.145
375	0.234	0.229	0.232
187.5	0.347	0.338	0.343
93.75	0.562	0.576	0.569
46.88	0.865	0.921	0.893
23.44	1.464	1.392	1.428
0	2.677	2.568	2.623

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	O09107

Applications	ELISA
Research Area	INSL3

Storage	Store at 2-8°C. Please refer to protocol.

Reference	The beneficial effects of betaine on dysfunctional adipose tissue and N6-methyladenosine mRNA methylation requires the AMP-activated protein kinase a1 subunit Xihong Zhou. et al,The Journal of Nutritional Biochemistry,Available online 20August 2015 Induction of tolerance and prolongation of islet allograft survival by syngeneic hematopoietic stem cell transplantation in mice Yang SF. et al,Transpl Immunol,2015 Aug 24.pii: S0966-3274(15)30016-2.

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SKU	Supplier No.	Size	Your price
BHE10505956	CSB-EL011747MO	96T	$595	Add to Cart
BHE10505956	CSB-EL011747MO	5x96T	$2856	Add to Cart
BHE10505956	CSB-EL011747MO	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.