Mouse Insulin,INS ELISA Kit

Mfr.No.

CSB-E05071m

Sensitivity

3.9 nIU/mL

Detection Range

15.6 nIU/mL-1000 nIU/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Insulin,INS

Recovery

The recovery of human LMTK3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-98
EDTA plasma (n=4)	105	100-110

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human LMTK3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-94
1:2	Average %	97
	Range %	92-101
1:4	Average %	89
	Range %	84-93
1:8	Average %	94
	Range %	90-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1200	2.687	2.532	2.610	2.450
600	1.994	2.018	2.006	1.846
300	1.269	1.295	1.282	1.122
150	0.716	0.728	0.722	0.562
75	0.475	0.483	0.479	0.319
37.5	0.321	0.307	0.314	0.154
18.75	0.243	0.239	0.241	0.081
0	0.158	0.162	0.160	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P01325

Applications	ELISA
Research Area	INS

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Combined Transfection of the Three Transcriptional Factors, PDX-1, NeuroD1, and MafA, Causes Differentiation of BoneMarrow Mesenchymal Stem Cells into Insulin … Guo Qing-Song et al,Experimental Diabetes Research,/ RIP140 mediates hyperglycemia-induced glucotoxicity in ß-cells via the activation of JNK and ERK1/2 signaling pathways Zou R et al,Diabetes Res Clin Pract,2013 Jan 2.pii: S0168-8227(12)00506-2. Antidiabetic drugs restore abnormal transport of amyloid-ß across the blood-brain barrier and memory impairment in db/db mice Chen F. et al,Neuropharmacology,2015 Jul 23.pii: S0028-3908(15)30030-7. The multimerization and secretion of adiponectin are regulated by TNF-alpha He Y. et al,Endocrine,2015 Sep 25. High-throughput sequencing reveals altered expression of hepatic microRNAs in nonalcoholic fatty liver disease-related fibrosis. Leti F, et al,Transl Res,2015 Sep;166(3):304-14. 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Mouse Insulin,INS ELISA Kit

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