Mouse inducible nitric oxide synthase,iNOS ELISA KIT

Mfr.No.

CSB-E08326m

Alternative Names

nitric oxide synthase 2, inducible, HEP-NOS, INOS, NOS, NOS2A, NOS, type II, nitric oxide synthase 2A, nitric oxide synthase 2A (inducible, hepatocytes), nitric oxide synthase, macrophage

Reactivity

Mouse

Detection Range

7.8 IU/mL-500 IU/mL

Sample Type

Tissue homogenates, Cell lysates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse IL4I1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	94-107
EDTA plasma (n=4)	98	90-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL4I1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	101
	Range %	92-105
1:2	Average %	94
	Range %	90-99
1:4	Average %	95
	Range %	87-98
1:8	Average %	92
	Range %	85-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	1.780	1.814	1.797	1.596
500	1.316	1.301	1.309	1.108
250	0.954	0.939	0.947	0.746
125	0.716	0.732	0.724	0.523
62.5	0.488	0.498	0.493	0.292
31.2	0.406	0.400	0.403	0.202
15.6	0.306	0.294	0.300	0.099
0	0.203	0.199	0.201	?

Species

Mus musculus

Sensitivity

1.95 IU/mL

Limit Of Detection

450 nm

UniProt ID	P29477
Antigen	Nitric Oxide Synthase 2, Inducible

Applications

ELISA

Research Area

NOS2

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price
BHE10505948	CSB-E08326m	96T	$695	Add to Cart
BHE10505948	CSB-E08326m	5x96T	$3336	Add to Cart
BHE10505948	CSB-E08326m	10x96T	$6602.50	Add to Cart

Mouse inducible nitric oxide synthase,iNOS ELISA KIT

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Mouse inducible nitric oxide synthase,iNOS ELISA KIT

Rate it now..!!