Mouse inducible nitric oxide synthase,iNOS ELISA KIT

Mfr.No.

CSB-E08326m

Sensitivity

1.95 IU/mL

Detection Range

7.8 IU/mL-500 IU/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

nitric oxide synthase 2, inducible, HEP-NOS, INOS, NOS, NOS2A, NOS, type II, nitric oxide synthase 2A, nitric oxide synthase 2A (inducible, hepatocytes), nitric oxide synthase, macrophage

Recovery

The recovery of mouse IL4I1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	94-107
EDTA plasma (n=4)	98	90-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL4I1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	101
	Range %	92-105
1:2	Average %	94
	Range %	90-99
1:4	Average %	95
	Range %	87-98
1:8	Average %	92
	Range %	85-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	1.780	1.814	1.797	1.596
500	1.316	1.301	1.309	1.108
250	0.954	0.939	0.947	0.746
125	0.716	0.732	0.724	0.523
62.5	0.488	0.498	0.493	0.292
31.2	0.406	0.400	0.403	0.202
15.6	0.306	0.294	0.300	0.099
0	0.203	0.199	0.201	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P29477

Applications	ELISA
Research Area	NOS2

Storage	Store at 2-8°C. Please refer to protocol.

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Mouse inducible nitric oxide synthase,iNOS ELISA KIT

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