Mouse Indian Hedgehog (IHH) ELISA kit

Mfr.No.

CSB-E16517m

Sensitivity

0.78 pg/mL

Detection Range

3.12 pg/mL-200 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Indian Hedgehog (IHH), BDA1, HHG2, Indian hedgehog homolog

Recovery

The recovery of mouse DEFA4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	95-104
EDTA plasma (n=4)	83	80-86

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse DEFA4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	85
	Range %	81-89
1:2	Average %	93
	Range %	90-96
1:4	Average %	88
	Range %	84-92
1:8	Average %	101
	Range %	97-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P97812

Applications	ELISA
Research Area	IHH

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10505946	CSB-E16517m	10x96T	$7505	Add to Cart