Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of IL-17F and the recovery rates were calculated by comparing the measured value to the expected amount of IL-17F in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-101
95
EDTA plasma(n=5)
86-102
94
heparin plasma(n=5)
91-104
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-17F and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
86-104%
86-103%
85-105%
EDTA plasma(n=5)
87-99%
82-98%
95-101%
heparin plasma(n=5)
80-98%
80-94%
83-100%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
UniProt ID
Q7TNI7
Applications
ELISA
Applications Notes
IL-17F ELISA Kit allows for the in vitro quantitative determination of IL-17F concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse IL-17F (Interleukin-17F) ELISA Kit