Matrices listed below were spiked with certain level of IL-10 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-10 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-102
95
EDTA plasma(n=5)
84-103
92
heparin plasma(n=5)
88-105
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-10 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
83-93%
85-99%
83-102%
EDTA plasma(n=5)
91-101%
93-101%
91-101%
heparin plasma(n=5)
94-99%
90-100%
94-96%
Sensitivity
< 1.875pg/ml
Notes
For Research Use Only
UniProt ID
P18893
Applications
ELISA
Applications Notes
IL-10 ELISA Kit allows for the in vitro quantitative determination of IL-10 concentrations in serum, plasma, tissue homogenates and other biological fluids.