Mouse IL-1 Alpha/IL-1F1/IL1A ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

Mfr.No.

EZ0391

Detection Range

4.69 pg/ml - 300 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	4.7	9.4	18.8	37.5	75	150	300
O.D.	0.046	0.112	0.142	0.240	0.430	0.760	1.270	1.871

Description

Mouse IL-1 Alpha/IL-1F1/IL1A ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Mouse Il1a in cell culture supeatants and serum.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S115-S270

Kit Components

Description
Capture Antibody
Detection Antibody
Avidin-Biotin-Peroxidase Complex (ABC)
Lyophilized Standard

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse Il1a

Sample Type

Cell culture supeatants, Serum

Background

Interleukin-1 alpha(IL-1 alpha) and interleukin-1 beta(IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair. IL-1 alpha has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases. Recently, it has been shown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors.1 The human interleukin 1 alpha gene is assigned to chromosome 2. Genetic polymorphisms at interleukin(IL-1alpha and IL-1beta) have been recently suggested to be associated with severity of adult periodontitis. The murine IL-1 alpha and IL-1 beta genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis. The standard product used in this kit is recombinant mouse IL-1alpha with the molecular mass of 18kDa.

Notes

Material Required But Not Provided:
1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
10. Substrate Solution: Tetramethylbenzidine
11. Stop Solution: 2 N H2SO4
12. Wash Buffer . Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
*Item 6 12 are included in the EZ Set Accessory Kit

Gene ID	16175
Gene Name	Il1a
UniProt ID	P01582

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21000132	EZ0391	5 plates/kit	$500		Add to Cart

Mouse IL-1 Alpha/IL-1F1/IL1A ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Mouse IL-1 Alpha/IL-1F1/IL1A ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

Rate it now..!!