Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of Ikbkb and the recovery rates were calculated by comparing the measured value to the expected amount of Ikbkb in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
93-103
99
EDTA plasma(n=5)
88-103
94
heparin plasma(n=5)
86-105
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ikbkb and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
90-104%
85-92%
87-103%
EDTA plasma(n=5)
89-100%
82-97%
83-98%
heparin plasma(n=5)
81-97%
80-93%
81-93%
Sensitivity
< 46.875pg/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
Ikbkb ELISA Kit allows for the in vitro quantitative determination of Ikbkb concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse Ikbkb (Inhibitor of nuclear factor kappa-B kinase subunit beta) ELISA Kit