Mouse IGFBP5 ELISA Kit PicoKine®

Mfr.No.

EK1183

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	156	312	625	125000	25000	5000	10000
O.D.	0.006	0.064	0.125	0.259	0.511	0.936	1.623	2.194

Description

Mouse IGFBP5 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Igfbp5 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Insulin-like growth factor-binding protein 5;IBP-5;IGF-binding protein 5;IGFBP-5;Igfbp5;Igfbp-5;

Molecular Weight

30372 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: L20-E271

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse IGFBP5

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	223	1270	6078	229	1195	6403
Standard deviation	16.5	71.12	257.4%7	21.29	76.48	332.95
CV (%)	7.4%	5.6%	4.2%	9.3%	6.4%	5.2%

Protein Function

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Background

Insulin-like growth factor-binding protein 5 is a protein that in humans is encoded by the IGFBP5 gene. The IGFBP5 gene was mapped to chromosome 2q33-q34. The expression of IGFBP5 by stable transfection and adenovirus-mediated infection was inhibitory to growth in 2 human breast cancer cell lines. Stable expression of IGFBP5 in the breast cancer cell lines also inhibited the formation and growth of tumors following injection in athymic mice. IGFBP5 is believed a growth inhibitor and proapoptotic agent in breast cancer cells.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	223	257	257	258	248	14.87	5.9%
Sample 2	1270	1408	1312	1242	1308	62.88	4.8%
Sample 3	6078	5394	5607	6353	5858	378.06	6.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	16011
Gene Name	IGFBP5
UniProt ID	Q07079
Protein Name	Insulin-like growth factor-binding protein 5
Tissue Specificity	Most abundant in kidney, uterus and gastrocnemius muscle.
Sequence	Contains 1 IGFBP N-terminal domain.

Applications	ELISA
Research Area	Growth Factors/Hormones,Lipid Signaling,Signal Transduction,Signaling Pathway

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000804	EK1183	96 wells/kit	$499		Add to Cart

Mouse IGFBP5 ELISA Kit PicoKine®

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