Matrices listed below were spiked with certain level of IARS and the recovery rates were calculated by comparing the measured value to the expected amount of IARS in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-103
94
EDTA plasma(n=5)
88-105
99
heparin plasma(n=5)
87-105
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IARS and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
IARS ELISA Kit allows for the in vitro quantitative determination of IARS concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse IARS(Isoleucyl tRNA Synthetase) ELISA Kit