Matrices listed below were spiked with certain level of IAA and the recovery rates were calculated by comparing the measured value to the expected amount of IAA in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-102
96
EDTA plasma(n=5)
86-105
95
heparin plasma(n=5)
87-104
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IAA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
IAA ELISA Kit allows for the in vitro quantitative determination of IAA concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse IAA(Insulin Autoantibodies) ELISA Kit