Matrices listed below were spiked with certain level of I-PTH and the recovery rates were calculated by comparing the measured value to the expected amount of I-PTH in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-103
95
EDTA plasma(n=5)
92-100
96
heparin plasma(n=5)
87-101
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of I-PTH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
I-PTH ELISA Kit allows for the in vitro quantitative determination of I-PTH concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse I-PTH(intact PaRathormone) ELISA Kit