Mouse hydroxyproline,Hyp ELISA Kit

Mfr.No.

CSB-E08839m

Sensitivity

1.95 ng/mL

Detection Range

7.8 ng/mL-500 ng/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

hydroxyproline,Hyp

Recovery

The recovery of human B4GALT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	80-90
EDTA plasma (n=4)	100	95-108

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human B4GALT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	96
	Range %	90-102
1:2	Average %	89
	Range %	84-95
1:4	Average %	91
	Range %	87-99
1:8	Average %	92
	Range %	88-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Applications	ELISA
Research Area	Hyp

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Metabolism, health and fillet nutritional quality in Atlantic salmon (Salmo salar) fed diets containing n-3-rich microalgae Katerina Kousoulaki. et al,Journal of Nutritional Science,2015 Jun 11;4:e24. Andrographolide Sodium Bisulfate Prevents UV-Induced Skin Photoaging through Inhibiting Oxidative Stress and Inflammation Janis Ya-Xian Zhan. et al,Mediators of Inflammation,2016 12 pages Flavonoid Compound Icariin Activates Hypoxia Inducible Factor-1伪 in Chondrocytes and Promotes Articular Cartilage Repair. Wang P. et al,PLoS One.,2016 11(2):e0148372. Synthesis, construction, and evaluation of self-assembled nano-bacitracin A as an efficient antibacterial agent in vitro and in vivo Hong W.et al,Int J Nanomedicine.,Jul 30

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