Mouse Hydroxyacid oxidase 1(HAO1) ELISA kit

Mfr.No.

CSB-EL010127MO

Alternative Names

hydroxyacid oxidase (glycolate oxidase) 1, GOX, GOX1, HAOX1, MGC142225, MGC142227, (S)-2-hydroxy-acid oxidase, glycolate oxidase, hydroxyacid oxidase 1

Reactivity

Mouse

Detection Range

6.25 pg/mL-400 pg/mL

Sample Type

Cell lysates, Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human MIOX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	89-100
EDTA plasma (n=4)	91	88-95

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human MIOX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	89
	Range %	84-92
1:2	Average %	102
	Range %	99-105
1:4	Average %	86
	Range %	81-90
1:8	Average %	98
	Range %	94-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1200	2.443	2.378	2.411	2.217
600	2.045	2.056	2.051	1.857
300	1.577	1.398	1.488	1.294
150	0.984	0.987	0.986	0.792
75	0.641	0.638	0.640	0.446
37.5	0.434	0.445	0.440	0.246
18.75	0.334	0.329	0.332	0.138
0	0.195	0.193	0.194	?

Species

Mus musculus

Sensitivity

1.56 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q9WU19
Antigen	hydroxyacid oxidase (glycolate oxidase) 1

Applications

ELISA

Research Area

HAO1

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10505931	CSB-EL010127MO	96T	$695	Add to Cart
BHE10505931	CSB-EL010127MO	5x96T	$3336	Add to Cart
BHE10505931	CSB-EL010127MO	10x96T	$6602.50	Add to Cart

Mouse Hydroxyacid oxidase 1(HAO1) ELISA kit

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