Mouse Hyaluronan Synthase 3

Mfr.No.

E1219Mo

Sensitivity

9.31ng/L

Detection Range

20-3800ng/L

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Mouse Hyaluronan Synthase 3 (also known as HAS3) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

HA Synthase 3;HAS3;Hyaluronan Synthase 3;Hyaluronate Synthase 3;Hyaluronic acid Synthase 3

Molecular Weight

63349

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1219Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	345.37	13.5	3.91
Intra-Assay	2	18	1258.32	34.88	2.77
Intra-Assay	3	18	759.45	36.49	4.8

Background

Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer. Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation. This is one of the isozymes catalyzing that reaction.

Protocol

These standard curves of E1219Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2000ng/L	2.319	2.27	2.249
	2.22
1000ng/L	1.404	1.396	1.375
	1.389
500ng/L	0.725	0.718	0.697
	0.71
250ng/L	0.303	0.3	0.279
	0.297
125ng/L	0.123	0.133	0.112
	0.143
0ng/L	0.019	0.021	0
	0.023

Gene ID	15118
UniProt ID	O08650

Research Area	Tags & Cell Markers

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12109084	E1219Mo	48T	$320		Add to Cart
BHE12109084	E1219Mo	96T	$458		Add to Cart

Mouse Hyaluronan Synthase 3 - HAS3 ELISA Kit

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