Mouse Hyaluronan Synthase 1

Mfr.No.

E2139Mo

Sensitivity

1.53ng/L

Detection Range

3-900ng/L

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

HA Synthase 1;HAS;HAS1;Hyaluronan Synthase 1;Hyaluronate Synthase 1;Hyaluronic acid Synthase 1

Molecular Weight

65545

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2139Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	174.19	6.17	3.54
Intra-Assay	2	18	246.22	10.1	4.1
Intra-Assay	3	18	362.32	10.82	2.99

Background

Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer. Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation. This is one of the isozymes catalyzing that reaction. Also able to catalyze the synthesis of chito-oligosaccharide depending on the substrate.

Protocol

These standard curves of E2139Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
480ng/L	2.116	2.126	2.082
	2.135
240ng/L	0.926	1.027	0.983
	1.128
120ng/L	0.527	0.515	0.471
	0.502
60ng/L	0.184	0.19	0.146
	0.195
30ng/L	0.098	0.096	0.052
	0.094
0ng/L	0.046	0.044	0
	0.041

Gene ID	15116
UniProt ID	Q61647

Research Area	Cardiovascular

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12109950	E2139Mo	48T	$320		Add to Cart
BHE12109950	E2139Mo	96T	$458		Add to Cart

Mouse Hyaluronan Synthase 1 - HAS1 ELISA Kit

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