Matrices listed below were spiked with certain level of Htra4 and the recovery rates were calculated by comparing the measured value to the expected amount of Htra4 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
82-105
99
EDTA plasma(n=5)
81-101
91
heparin plasma(n=5)
86-105
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Htra4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-102%
94-97%
82-101%
EDTA plasma(n=5)
91-99%
92-101%
90-101%
heparin plasma(n=5)
93-100%
90-96%
90-98%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
UniProt ID
A2RT60
Applications
ELISA
Applications Notes
HtrA4ELISA Kit allows for the in vitro quantitative determination of Htra4 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse HtrA4(Serine protease HTRA4)ELISA Kit