Mouse HSP-27(heat shock protein 27) ELISA Kit

Mfr.No.

EM1135

Alternative Names

HSP27 ELISA Kit, Heat Shock Protein 27 ELISA Kit, HSP-27 ELISA Kit, HSPB1 ELISA Kit

Reactivity

Mouse

Detection Method

Double Antibody

Detection Range

0.625-40ng/ml

Sample Type

Cell lysate, Tissue lysate, Other liquid samples, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of HSP-27 and the recovery rates were calculated by comparing the measured value to the expected amount of HSP-27 in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	89-103	97
EDTA plasma(n=5)	87-102	92
heparin plasma(n=5)	85-100	92

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HSP-27 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	89-102%	88-102%	86-100%
EDTA plasma(n=5)	86-98%	87-101%	89-99%
heparin plasma(n=5)	89-98%	82-94%	81-95%

Sensitivity

< 0.375ng/ml

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Background

HSP27(Heat Shock Protein 27)/HSP-27/HSPB1

Notes

For Research Use Only

UniProt ID	P14602
Antigen	HSP-27

Applications	ELISA
Applications Notes	HSP-27 ELISA Kit allows for the in vitro quantitative determination of HSP-27 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10800118	EM1135	96T	$350		Add to Cart

Mouse HSP-27(heat shock protein 27) ELISA Kit

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