Mouse Homocysteine(HCY)ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E16551m

Sensitivity

1.1 nmol/mL

Detection Range

4.7 nmol/mL-300 nmol/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Homocysteine(HCY)

Recovery

The recovery of rat HA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	90-96
EDTA plasma (n=4)	95	90-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat HA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	93
	Range %	90-96
1:2	Average %	91
	Range %	87-96
1:4	Average %	97
	Range %	93-100
1:8	Average %	89
	Range %	85-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	Hcy

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Role of osteocytes in mediating bone mineralization during hyperhomocysteinemia Vijayan V.et al,J Endocrinol,Jun;233(3):243-255.

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