Mouse Homocysteic acid,Hcy ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08897m

Sensitivity

3.9 pmol/mL

Detection Range

15.6 pmol/mL-1000 pmol/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Homocysteic acid,Hcy

Recovery

The recovery of bovine SP-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-93
EDTA plasma (n=4)	106	103-108

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine SP-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-103
1:2	Average %	91
	Range %	85-97
1:4	Average %	90
	Range %	88-94
1:8	Average %	100
	Range %	96-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	Hcy

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Folic acid prevents cardiac dysfunction and reduces myocardial fibrosis in a mouse model of high-fat diet-induced obesity Li W.et al,Nutr Metab (Lond),Nov 2

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