Mouse Homocysteic acid,Hcy ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08897m

Sensitivity

3.9 pmol/mL

Detection Range

15.6 pmol/mL-1000 pmol/mL

Reactivity

Mouse

Sample Type

Serum, Plasma

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Homocysteic acid,Hcy

Recovery

The recovery of bovine SP-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-93
EDTA plasma (n=4)	106	103-108

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine SP-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-103
1:2	Average %	91
	Range %	85-97
1:4	Average %	90
	Range %	88-94
1:8	Average %	100
	Range %	96-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

Antigen	Homocysteic acid,Hcy

Applications	ELISA
Research Area	Hcy

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Folic acid prevents cardiac dysfunction and reduces myocardial fibrosis in a mouse model of high-fat diet-induced obesity Li W.et al,Nutr Metab (Lond),Nov 2

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