Matrices listed below were spiked with certain level of Hn1l and the recovery rates were calculated by comparing the measured value to the expected amount of Hn1l in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-101
91
EDTA plasma(n=5)
85-95
90
heparin plasma(n=5)
88-94
91
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Hn1l and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
88-103%
91-101%
92-101%
EDTA plasma(n=5)
86-100%
86-98%
97-101%
heparin plasma(n=5)
80-96%
93-100%
87-97%
Sensitivity
< 46.875pg/ml
Notes
For Research Use Only
UniProt ID
Q6PGH2
Applications
ELISA
Applications Notes
Hn1l ELISA Kit allows for the in vitro quantitative determination of Hn1l concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse Hn1l (Hematological and neurological expressed 1-like protein) ELISA Kit