Matrices listed below were spiked with certain level of HK1 and the recovery rates were calculated by comparing the measured value to the expected amount of HK1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-99
94
EDTA plasma(n=5)
88-101
93
heparin plasma(n=5)
85-105
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HK1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
HK1 ELISA Kit allows for the in vitro quantitative determination of HK1 concentrations in serum, plasma, tissue homogenates and other biological fluids.