Mouse Histone deacetylase complex subunit SAP130(SAP130) ELISA kit

Mfr.No.

CSB-EL020694MO

Alternative Names

Sin3A-associated protein, 130kDa, FLJ12761, Sin3-associated polypeptide p130, histone deacetylase complex subunit SAP130, mSin3A-associated protein 130

Reactivity

Mouse

Detection Range

46.88 pg/mL-3000 pg/mL

Sample Type

Cell lysates, Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human IGF2BP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	84-93
EDTA plasma (n=4)	95	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IGF2BP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-90
1:2	Average %	96
	Range %	92-100
1:4	Average %	90
	Range %	85-94
1:8	Average %	104
	Range %	100-108

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
8000	2.167	2.249	2.208	2.051
4000	1.649	1.703	1.676	1.519
2000	1.174	1.114	1.144	0.987
1000	0.791	0.758	0.775	0.618
500	0.458	0.472	0.465	0.308
250	0.335	0.347	0.341	0.184
125	0.226	0.236	0.231	0.074
0	0.153	0.160	0.157	?

Species

Mus musculus

Sensitivity

11.72 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q8BIH0
Antigen	Sin3A-associated protein, 130kDa

Applications

ELISA

Research Area

SAP130

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10505917	CSB-EL020694MO	96T	$695	Add to Cart
BHE10505917	CSB-EL020694MO	5x96T	$3336	Add to Cart
BHE10505917	CSB-EL020694MO	10x96T	$6602.50	Add to Cart

Mouse Histone deacetylase complex subunit SAP130(SAP130) ELISA kit

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