Matrices listed below were spiked with certain level of histon-H2b and the recovery rates were calculated by comparing the measured value to the expected amount of histon-H2b in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
91-105
97
EDTA plasma(n=5)
85-103
97
heparin plasma(n=5)
87-100
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of histon-H2b and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
histon-H2b ELISA Kit allows for the in vitro quantitative determination of histon-H2b concentrations in serum, plasma, tissue homogenates and other biological fluids.