Mouse Histidine-rich glycoprotein(HRG) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EL010736MO

Alternative Names

histidine-rich glycoprotein, DKFZp779H1622, HPRG, HRGP, histidine-proline rich glycoprotein

Reactivity

Mouse

Detection Range

6.25 ng/mL-400 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of mouse TNF-? spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	90	85-95

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse TNF-? in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-100
1:2	Average %	105
	Range %	100-110
1:4	Average %	92
	Range %	88-100
1:8	Average %	84
	Range %	80-88

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Sensitivity

3.12 ng/mL

Limit Of Detection

450 nm

UniProt ID	Q9ESB3
Antigen	histidine-rich glycoprotein

Applications

ELISA

Research Area

HRG

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10505915	CSB-EL010736MO	96T	$695	Add to Cart
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BHE10505915	CSB-EL010736MO	10x96T	$6602.50	Add to Cart