Matrices listed below were spiked with certain level of His and the recovery rates were calculated by comparing the measured value to the expected amount of His in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
91-102
96
EDTA plasma(n=5)
88-104
96
heparin plasma(n=5)
89-101
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of His and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
His ELISA Kit allows for the in vitro quantitative determination of His concentrations in serum, plasma, tissue homogenates and other biological fluids.