Matrices listed below were spiked with certain level of HHIP and the recovery rates were calculated by comparing the measured value to the expected amount of HHIP in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-102
96
EDTA plasma(n=5)
87-104
96
heparin plasma(n=5)
86-104
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HHIP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
HHIP ELISA Kit allows for the in vitro quantitative determination of HHIP concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse HHIP(Hedgehog Interacting Protein) ELISA Kit