Matrices listed below were spiked with certain level of Hes1 and the recovery rates were calculated by comparing the measured value to the expected amount of Hes1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-100
94
EDTA plasma(n=5)
86-103
99
heparin plasma(n=5)
85-105
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Hes1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-98%
97-105%
86-105%
EDTA plasma(n=5)
88-94%
83-98%
82-92%
heparin plasma(n=5)
80-91%
82-95%
82-97%
Sensitivity
< 46.875pg/ml
Notes
For Research Use Only
UniProt ID
P35428
Applications
ELISA
Applications Notes
Hes1 ELISA Kit allows for the in vitro quantitative determination of Hes1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse Hes1 (Transcription factor HES-1) ELISA Kit