Mouse Hepatocyte Growth Factor Receptor(C-MET/HGFR)ELISA Kit

Mouse Hepatocyte Growth Factor Receptor(C-MET/HGFR)ELISA Kit

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Mfr.No.

CSB-E13492m

Sensitivity

0.02 ng/mL

Detection Range

0.078 ng/mL-5 ng/mL

Reactivity

Mouse

Assay Duration

1-5h

Sample Volume

50-100ul

Alternative Names

met proto-oncogene (hepatocyte growth factor receptor), AUTS9, HGFR, RCCP2, c-Met, HGF receptor, SF receptor, met proto-oncogene, met proto-oncogene tyrosine kinase, oncogene MET, scatter factor receptor

Recovery

The recovery of rat vWF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-93
EDTA plasma (n=4)	95	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat vWF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	91
	Range %	86-99
1:200	Average %	97
	Range %	92-105
1:400	Average %	85
	Range %	81-87
1:800	Average %	88
	Range %	83-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.300	2.488	2.394	2.257
10	2.011	2.102	2.057	1.920
5	1.446	1.560	1.503	1.366
2.5	0.903	0.900	0.902	0.765
1.25	0.628	0.586	0.607	0.470
0.625	0.350	0.364	0.357	0.220
0.312	0.242	0.258	0.250	0.113
0	0.134	0.140	0.137	?

Species

Mus musculus

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P16056

Applications	ELISA
Research Area	MET

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Hepatocyte growth factor (HGF) optimizes oral traumatic ulcer healing of mice by reducing inflammation Wang X.et al,Cytokine,Aug 19 Novel aminochromone derivative inhibits tumor growth on xenograft model of lung cancer in mice Ekaterina V Blinova, et al,Joual of Advanced pharmaceutical technology research,December 10

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SKU	Supplier No.	Size	Your price
BHE10505903	CSB-E13492m	96T	$595	Add to Cart
BHE10505903	CSB-E13492m	5x96T	$2856	Add to Cart
BHE10505903	CSB-E13492m	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.