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Home / ELISA Kit

Mouse Hepatocyte growth factor activator (HGFAC) ELISA Kit

SKU : BHE13706641

HGFAC belongs to peptidase family S1. It is first synthesized as an inactive single-chain precursor before being activated to a heterodimeric form by endoproteolytic processing. It acts as serine protease that converts hepatocyte growth factor to the active form. Miyazawa et al. (1993) determined the partial amino acid sequence of the homologous human serine protease and used it to design degenerate primers to screen a human liver cDNA library. They identified a 655-amino acid inactive precursor protein, designated hepatocyte growth factor activator (HGFAC), with a calculated molecular mass of 70,681 Da. They determined that the active protein purified from serum is derived from the C terminus of the precursor by proteolytic cleavage of the bonds between arg372-val373 and arg407-ile408.

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Mfr.No.	KTE71484
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Hepatocyte growth factor activator (HGFAC) ELISA Kit employs a two-site sandwich ELISA to quantitate HGFAC.
Alternative Names	HGFAC; RP11-529E10.2; HGFA; MGC138395; MGC138397;
Detection Method	Colorimetric
Kit Components	Mouse Hepatocyte growth factor activator microplate Mouse Hepatocyte growth factor activator standard Mouse Hepatocyte growth factor activator detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Hepatocyte growth factor activator (HGFAC) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse HGFAC. No significant cross-reactivity or interference between Mouse HGFAC and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	HGFAC belongs to peptidase family S1. It is first synthesized as an inactive single-chain precursor before being activated to a heterodimeric form by endoproteolytic processing. It acts as serine protease that converts hepatocyte growth factor to the active form. Miyazawa et al. (1993) determined the partial amino acid sequence of the homologous human serine protease and used it to design degenerate primers to screen a human liver cDNA library. They identified a 655-amino acid inactive precursor protein, designated hepatocyte growth factor activator (HGFAC), with a calculated molecular mass of 70,681 Da. They determined that the active protein purified from serum is derived from the C terminus of the precursor by proteolytic cleavage of the bonds between arg372-val373 and arg407-ile408.
Notes	This Mouse Hepatocyte growth factor activator (HGFAC) ELISA Kit employs a two-site sandwich ELISA to quantitate HGFAC in samples. An antibody specific for HGFAC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHGFAC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HGFAC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HGFAC bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	54426

Applications	ELISA
Applications Notes	This Mouse Hepatocyte growth factor activator (HGFAC) ELISA Kit employs a two-site sandwich ELISA to quantitate HGFAC in samples. An antibody specific for HGFAC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHGFAC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HGFAC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HGFAC bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13706641	KTE71484	48 T	$339		Add to Cart
BHE13706641	KTE71484	96 T	$589		Add to Cart
BHE13706641	KTE71484	96 Tx5	$2629		Add to Cart
BHE13706641	KTE71484	96 Tx50	$24569		Add to Cart

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