Mouse Hemojuvelin/RGM-C/HJV ELISA Kit PicoKine®

Mfr.No.

EK1370

Sensitivity

<10 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.055	0.137	0.182	0.295	0.496	0.897	1.563	2.225

Description

Mouse Hemojuvelin/RGM-C/HJV ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Hjv in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Hemojuvelin;Hemochromatosis type 2 protein homolog;RGM domain family member C;Hfe2;Rgmc;

Molecular Weight

44848 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q33-D393

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse Hemojuvelin

Subcellular Localization

Cell membrane ; Lipid-anchor, GPI-anchor . Also released in the extracellular space. .

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	174	404	1769	170	398	1835
Standard deviation	11.48	18.98	714.453	14.45	25.87	114.7%
CV (%)	6.6%	4.7%	4.4%	8.5%	6.5%	6.3%

Protein Function

Acts as a bone morphogenetic protein (BMP) coreceptor. Through enhancement of BMP signaling regulates hepcidin (HAMP) expression and regulates iron homeostasis. .

Background

Hemojuvelin (HJV/RGMc/HFE2) is a membrane-bound and soluble protein in mammals. It is a member of a three gene family (in vertebrates) called the repulsive guidance molecules. In mouse, the Hemojuvelin protein is encoded by the HFE2 gene, and it is mapped to Chromosome 3. It was concluded that downregulation of hepatic Hemojuvelin during inflammation may induce a temporary elimination of iron sensing. Hemojuvelin is a coreceptor for bone morphogenetic proteins (BMPs), and inhibition of endogenous BMP signaling reduces hepcidin expression and increases serum iron in mice. Furthermore, Hemojuvelin may play inhibitory roles in prostate cancer by suppressing cell growth, adhesion, migration and invasion.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	174	203	203	206	196	13.04	6.6%
Sample 2	404	461	449	447	440	21.6	4.9%
Sample 3	1769	1736	1928	1768	1800	74.94	4.1%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	69585
Gene Name	HFE2
UniProt ID	Q7TQ32
Protein Name	Hemojuvelin
Tissue Specificity	Muscle cell lineage. .

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000960	EK1370	96 wells/kit	$499		Add to Cart

Mouse Hemojuvelin/RGM-C/HJV ELISA Kit PicoKine®

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