Mouse heme oxygenase 1,HO-1 ELISA Kit

Mfr.No.

CSB-E08268m

Alternative Names

heme oxygenase (decycling) 1, HO-1, HSP32, bK286B10, heme oxygenase (decyclizing) 1

Reactivity

Mouse

Detection Range

31.25 pg/mL-2000 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of dog ?2-MG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	86-99
EDTA plasma (n=4)	95	91-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of dog ?2-MG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	90
	Range %	83-99
1:2	Average %	92
	Range %	85-100
1:4	Average %	95
	Range %	85-105
1:8	Average %	95
	Range %	88-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Sensitivity

7.8 pg/mL

Limit Of Detection

450 nm

UniProt ID	P14901
Antigen	heme oxygenase (decycling) 1

Applications

ELISA

Research Area

HMOX1

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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