Matrices listed below were spiked with certain level of HCY and the recovery rates were calculated by comparing the measured value to the expected amount of HCY in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-98
92
EDTA plasma(n=5)
88-103
98
heparin plasma(n=5)
92-101
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HCY and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
HCY ELISA Kit allows for the in vitro quantitative determination of HCY concentrations in serum, plasma, tissue homogenates and other biological fluids.