Histone acetyltransferase type B catalytic subunit ELISA Kit
Reactivity
Mouse
Detection Method
Double Antibody
Detection Range
15.625-1000pg/ml
Sample Type
Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of Hat1 and the recovery rates were calculated by comparing the measured value to the expected amount of Hat1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-102
92
EDTA plasma(n=5)
85-105
92
heparin plasma(n=5)
87-104
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Hat1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
89-105%
88-99%
89-100%
EDTA plasma(n=5)
89-101%
82-101%
82-99%
heparin plasma(n=5)
85-100%
83-100%
82-100%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
Antigen
Hat1
Applications
ELISA
Applications Notes
Hat1 ELISA Kit allows for the in vitro quantitative determination of Hat1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
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