Matrices listed below were spiked with certain level of Has2 and the recovery rates were calculated by comparing the measured value to the expected amount of Has2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-103
95
EDTA plasma(n=5)
94-104
98
heparin plasma(n=5)
86-101
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Has2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Has2 ELISA Kit allows for the in vitro quantitative determination of Has2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse Has2(Hyaluronan synthase 2) ELISA Kit