Mouse H2afx(Histone H2A.x) ELISA Kit

Mfr.No.

EM0637

Alternative Names

H2afx ELISA Kit, H2a ELISA Kit, x ELISA Kit, H2AFX ELISA Kit, H2AX ELISA Kit, Histone H2A.X ELISA Kit

Reactivity

Mouse

Detection Method

Double Antibody

Detection Range

31.25-2000pg/ml

Sample Type

Other liquid samples, Cell lysate

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of H2afx and the recovery rates were calculated by comparing the measured value to the expected amount of H2afx in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	94-103	99
EDTA plasma(n=5)	90-104	97
heparin plasma(n=5)	93-105	100

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of H2afx and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	90-102%	86-103%	91-105%
EDTA plasma(n=5)	84-98%	82-90%	84-99%
heparin plasma(n=5)	81-98%	80-96%	80-99%

Sensitivity

< 18.75pg/ml

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Background

H2afx/H2a/x/H2AFX/H2AX/Histone H2A.X

Notes

For Research Use Only

UniProt ID	P27661
Antigen	H2AFX

Applications	ELISA
Applications Notes	H2afx ELISA Kit allows for the in vitro quantitative determination of H2afx concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10804362	EM0637	96T	$400		Add to Cart

Mouse H2afx (Histone H2A.x) ELISA Kit

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