Matrices listed below were spiked with certain level of H1-4 and the recovery rates were calculated by comparing the measured value to the expected amount of H1-4 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
91-103
97
EDTA plasma(n=5)
83-103
93
heparin plasma(n=5)
85-105
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of H1-4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
94-102%
80-101%
87-96%
EDTA plasma(n=5)
90-101%
90-101%
93-101%
heparin plasma(n=5)
90-99%
90-98%
91-99%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
P43274
Applications
ELISA
Applications Notes
H1-4 ELISA Kit allows for the in vitro quantitative determination of H1-4 concentrations in serum, plasma, tissue homogenates and other biological fluids.