Matrices listed below were spiked with certain level of GS and the recovery rates were calculated by comparing the measured value to the expected amount of GS in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-104
97
EDTA plasma(n=5)
90-101
95
heparin plasma(n=5)
90-102
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GS and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-97%
85-104%
90-96%
EDTA plasma(n=5)
85-100%
82-90%
89-101%
heparin plasma(n=5)
86-100%
81-100%
84-100%
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Notes
For Research Use Only
UniProt ID
P15105
Applications
ELISA
Applications Notes
GS ELISA Kit allows for the in vitro quantitative determination of GS concentrations in serum, plasma, tissue homogenates and other biological fluids.
Storage
2-8 °C for 6 months
Reviews of Mouse GS (Glutamine synthetase) ELISA Kit